Skills

  • Molecular Biology and Genetic Engineering – PCR, DNA sequencing, immunofluorescence, immunoblotting, flow cytometry (FACS in particular), ELISPOT for IFN-γ, H&E staining, molecular cloning, DNA/RNA/plasmid purification, CRISPR guide RNA design, in vitro transcription, T7E1 mismatch digesting assay, in vitro Cas9 digest assay, bacterial culture.
  • Tissue Engineering – mammalian cell culture (primary immune cells, tumor cell lines); lipofection, electroporation, and nucelofection of plasmids and ribonucleoprotein complexes; in vitro immune cell study – macrophage phagocytosis, T cell priming and proliferation.
  • In vivo – tumor induction in BALB/c and C57BL/6 mice by subcutaneous, intraperitoneal, and intravenous injections; submandibular and saphenous vein blood collection; organ harvesting (spleen, bone marrow, tumors); isolation of macrophages; naïve and primed T cells (CD4+/CD8+) from PBMCs and spleen.
  • Bioinformatics – PCR and sequencing primer design, CRISPR gRNA design, molecular protein docking, electropherogram analysis.

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